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cmv nls r geco  (Addgene inc)


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    Addgene inc cmv nls r geco
    Cmv Nls R Geco, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cmv+nls+r+geco/CMV-NLS-R-GECO+(Plasmid+%2332462)/pmc11872787__ALR___15___287___s001-9-6-16
    Average 92 stars, based on 14 article reviews
    cmv nls r geco - by Bioz Stars, 2026-11
    92/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Soluble cyclase-mediated nuclear cAMP synthesis is sufficient for cell proliferation.
    Article Snippet: K44A HA-dynamin 1 pcDNA3.1 was a gift from Dr. Sandra Schmid (Addgene plasmid # 34683). .. CMV-NLS-R-GECO was a gift from Robert Campbell (Addgene plasmid #32462). pcDNA5-TO-PV-NES-DsRed (PV-NES; Addgene plasmid #16339) and pcDNA5-TO-PV-NLS-Dsred (PV-NLS; Addgene plasmid #16341) were a gift from Anton Bennet. ..

    Article Title: Modular assembly of synthetic proteins that span the plasma membrane in mammalian cells
    Article Snippet: .. The fragment R-GECO was amplified from CMV-NLS-R-GECO, which was a gift from Robert Campbell (Addgene plasmid #32462) and inserted into our cassette subcloning methodology. .. All plasmids were transformed in Escherichia coli and were isolated using the Mini-prep kit (Invitrogen).

    Article Title: Hops bitter β‐acids have antibacterial effects against sinonasal Staphylococcus aureus but also induce sinonasal cilia and mitochondrial dysfunction
    Article Snippet: Rottlerin, Calphostin C, carboxy-PTIO, TMRE Mitochondrial Membrane Potential Assay Kit, U73122 and U73343 were purchased from Cayman (Ann Arbor, Michigan, USA). .. CMV-ER-LAR-GECO1 (Plasmid #61244), CMV-mito-LAR-GECO1.2 (Plasmid #61245), CMV-NLS-R-GECO (Plasmid #32462), and AMPKAR (Plasmid #35097) were purchased from Addgene (Watertown, Massachusetts, USA). .. LF1 was obtained from Enamine (Frankfurt am Main, Germany).

    Amplification:

    Article Title: Modular assembly of synthetic proteins that span the plasma membrane in mammalian cells
    Article Snippet: .. The fragment R-GECO was amplified from CMV-NLS-R-GECO, which was a gift from Robert Campbell (Addgene plasmid #32462) and inserted into our cassette subcloning methodology. .. All plasmids were transformed in Escherichia coli and were isolated using the Mini-prep kit (Invitrogen).

    Subcloning:

    Article Title: Modular assembly of synthetic proteins that span the plasma membrane in mammalian cells
    Article Snippet: .. The fragment R-GECO was amplified from CMV-NLS-R-GECO, which was a gift from Robert Campbell (Addgene plasmid #32462) and inserted into our cassette subcloning methodology. .. All plasmids were transformed in Escherichia coli and were isolated using the Mini-prep kit (Invitrogen).



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    Fig. 3 Nuclear CD38-mediated ADPR is essential for glucagon-induced nuclear Ca2+ signals. a, b Hepatocytes were infected with GCaMP6m and <t>NLS-R-GECO</t> adenoviruses, and Ca2+ signals were recorded. The time point where 100 nM glucagon (Gcg) is added is indicated by the arrow. Scale bar, 10 μm. c Cytosolic and nuclear Ca2+ responses to glucagon after preincubation with BAPTA-am (10 μM). d–f Cytosolic and nuclear Ca2+ responses to glucagon after incubation with various inhibitors of Ca2+ second messengers [8-Br-cADPR (100 μM, d), 8-Br- ADPR (100 μM, e), or Ned19 (10 μM, f)]. g, h Cytosolic and nuclear Ca2+ responses to glucagon after overexpression of nCD38 using wtCd38- NLS adenovirus in Cd38+/+ hepatocytes (g) or Cd38−/−hepatocytes (h). n = 20 cells for each condition in b–h.
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    Fig. 3 Nuclear CD38-mediated ADPR is essential for glucagon-induced nuclear Ca2+ signals. a, b Hepatocytes were infected with GCaMP6m and <t>NLS-R-GECO</t> adenoviruses, and Ca2+ signals were recorded. The time point where 100 nM glucagon (Gcg) is added is indicated by the arrow. Scale bar, 10 μm. c Cytosolic and nuclear Ca2+ responses to glucagon after preincubation with BAPTA-am (10 μM). d–f Cytosolic and nuclear Ca2+ responses to glucagon after incubation with various inhibitors of Ca2+ second messengers [8-Br-cADPR (100 μM, d), 8-Br- ADPR (100 μM, e), or Ned19 (10 μM, f)]. g, h Cytosolic and nuclear Ca2+ responses to glucagon after overexpression of nCD38 using wtCd38- NLS adenovirus in Cd38+/+ hepatocytes (g) or Cd38−/−hepatocytes (h). n = 20 cells for each condition in b–h.
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    Fig. 3 Nuclear CD38-mediated ADPR is essential for glucagon-induced nuclear Ca2+ signals. a, b Hepatocytes were infected with GCaMP6m and <t>NLS-R-GECO</t> adenoviruses, and Ca2+ signals were recorded. The time point where 100 nM glucagon (Gcg) is added is indicated by the arrow. Scale bar, 10 μm. c Cytosolic and nuclear Ca2+ responses to glucagon after preincubation with BAPTA-am (10 μM). d–f Cytosolic and nuclear Ca2+ responses to glucagon after incubation with various inhibitors of Ca2+ second messengers [8-Br-cADPR (100 μM, d), 8-Br- ADPR (100 μM, e), or Ned19 (10 μM, f)]. g, h Cytosolic and nuclear Ca2+ responses to glucagon after overexpression of nCD38 using wtCd38- NLS adenovirus in Cd38+/+ hepatocytes (g) or Cd38−/−hepatocytes (h). n = 20 cells for each condition in b–h.
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    Image Search Results


    Fig. 3 Nuclear CD38-mediated ADPR is essential for glucagon-induced nuclear Ca2+ signals. a, b Hepatocytes were infected with GCaMP6m and NLS-R-GECO adenoviruses, and Ca2+ signals were recorded. The time point where 100 nM glucagon (Gcg) is added is indicated by the arrow. Scale bar, 10 μm. c Cytosolic and nuclear Ca2+ responses to glucagon after preincubation with BAPTA-am (10 μM). d–f Cytosolic and nuclear Ca2+ responses to glucagon after incubation with various inhibitors of Ca2+ second messengers [8-Br-cADPR (100 μM, d), 8-Br- ADPR (100 μM, e), or Ned19 (10 μM, f)]. g, h Cytosolic and nuclear Ca2+ responses to glucagon after overexpression of nCD38 using wtCd38- NLS adenovirus in Cd38+/+ hepatocytes (g) or Cd38−/−hepatocytes (h). n = 20 cells for each condition in b–h.

    Journal: Experimental & molecular medicine

    Article Title: CD38/ADP-ribose/TRPM2-mediated nuclear Ca 2+ signaling is essential for hepatic gluconeogenesis in fasting and diabetes.

    doi: 10.1038/s12276-023-01034-9

    Figure Lengend Snippet: Fig. 3 Nuclear CD38-mediated ADPR is essential for glucagon-induced nuclear Ca2+ signals. a, b Hepatocytes were infected with GCaMP6m and NLS-R-GECO adenoviruses, and Ca2+ signals were recorded. The time point where 100 nM glucagon (Gcg) is added is indicated by the arrow. Scale bar, 10 μm. c Cytosolic and nuclear Ca2+ responses to glucagon after preincubation with BAPTA-am (10 μM). d–f Cytosolic and nuclear Ca2+ responses to glucagon after incubation with various inhibitors of Ca2+ second messengers [8-Br-cADPR (100 μM, d), 8-Br- ADPR (100 μM, e), or Ned19 (10 μM, f)]. g, h Cytosolic and nuclear Ca2+ responses to glucagon after overexpression of nCD38 using wtCd38- NLS adenovirus in Cd38+/+ hepatocytes (g) or Cd38−/−hepatocytes (h). n = 20 cells for each condition in b–h.

    Article Snippet: Generation of NLS-R GECO Adenovirus: NLS R GECO adenovirus was constructed using NLS R GECO plasmid (Addgene, 32462)19.

    Techniques: Infection, Incubation, Over Expression